Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies

Teresa Cruz-Bustos; Marlies Dolezal; Anna Sophia Feix; Bärbel Ruttkowski; Karin Hummel; Ebrahim Razzazi-Fazeli; Anja Joachim

doi:10.3389/fcimb.2023.1271731

Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies

Front Cell Infect Microbiol. 2023 Oct 25:13:1271731. doi: 10.3389/fcimb.2023.1271731. eCollection 2023.

Authors

Teresa Cruz-Bustos¹, Marlies Dolezal², Anna Sophia Feix¹, Bärbel Ruttkowski¹, Karin Hummel³, Ebrahim Razzazi-Fazeli³, Anja Joachim¹

Affiliations

¹ Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria.
² Platform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria.
³ VetCore Facility (Proteomics), University of Veterinary Medicine Vienna, Vienna, Austria.

Abstract

Introduction: The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.

Methods: To advance our understanding of the parasite's cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.

Results: A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.

Discussion: These findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.

Keywords: Apicomplexa; Isospora suis; coccidia; gamonts; metabolism; oocyst; proteome; sexual development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Developmental Biology
Life Cycle Stages
Oocysts
Parasites*
Swine
Toxoplasma*

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Austrian Science Fund (FWF), grant number: P 33123-B.