A strong case for third-party testing

doi:10.7554/eLife.93329

Editorial

. 2023 Nov 14:12:e93329.

doi: 10.7554/eLife.93329.

A strong case for third-party testing

Fridtjof Lund-Johansen¹

Affiliations

PMID: 37962204
PMCID: PMC10645416
DOI: 10.7554/eLife.93329

Editorial

A strong case for third-party testing

Fridtjof Lund-Johansen. Elife. 2023.

. 2023 Nov 14:12:e93329.

doi: 10.7554/eLife.93329.

Author

Fridtjof Lund-Johansen¹

Affiliation

¹ Department of Immunology, Oslo University Hospital, Oslo, Norway.

PMID: 37962204
PMCID: PMC10645416
DOI: 10.7554/eLife.93329

Abstract

A strategy to identify high-quality commercially available antibodies for research reveals extensive use of non-specific antibodies and offers solutions for future large-scale testing.

Keywords: antibody; antibody specificity; antibody validation; biochemistry; cell biology; chemical biology; human; polyclonal antibody; recombinant antibody.

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Conflict of interest statement

FL No competing interests declared

Comment on

doi: 10.7554/eLife.91645

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References

1. Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S. Antibody performance in western blot applications is context-dependent. Biotechnology Journal. 2014;9:435–445. doi: 10.1002/biot.201300341. - DOI - PubMed
1. Andersson S, Sundberg M, Pristovsek N, Ibrahim A, Jonsson P, Katona B, Clausson CM, Zieba A, Ramström M, Söderberg O, Williams C, Asplund A. Insufficient antibody validation challenges oestrogen receptor beta research. Nature Communications. 2017;8:15840. doi: 10.1038/ncomms15840. - DOI - PMC - PubMed
1. Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Moleon VR, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown CM, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications. eLife. 2023;12:e91645. doi: 10.7554/eLife.91645.1. - DOI - PMC - PubMed
1. Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521:274–276. doi: 10.1038/521274a. - DOI - PubMed
1. Bradbury A, Plückthun A. Reproducibility: standardize antibodies used in research. Nature. 2015;518:27–29. doi: 10.1038/518027a. - DOI - PubMed

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[1] Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S. Antibody performance in western blot applications is context-dependent. Biotechnology Journal. 2014;9:435–445. doi: 10.1002/biot.201300341. - DOI - PubMed

[2] Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S. Antibody performance in western blot applications is context-dependent. Biotechnology Journal. 2014;9:435–445. doi: 10.1002/biot.201300341. - DOI - PubMed

[3] Andersson S, Sundberg M, Pristovsek N, Ibrahim A, Jonsson P, Katona B, Clausson CM, Zieba A, Ramström M, Söderberg O, Williams C, Asplund A. Insufficient antibody validation challenges oestrogen receptor beta research. Nature Communications. 2017;8:15840. doi: 10.1038/ncomms15840. - DOI - PMC - PubMed

[4] Andersson S, Sundberg M, Pristovsek N, Ibrahim A, Jonsson P, Katona B, Clausson CM, Zieba A, Ramström M, Söderberg O, Williams C, Asplund A. Insufficient antibody validation challenges oestrogen receptor beta research. Nature Communications. 2017;8:15840. doi: 10.1038/ncomms15840. - DOI - PMC - PubMed

[5] Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Moleon VR, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown CM, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications. eLife. 2023;12:e91645. doi: 10.7554/eLife.91645.1. - DOI - PMC - PubMed

[6] Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Moleon VR, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown CM, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications. eLife. 2023;12:e91645. doi: 10.7554/eLife.91645.1. - DOI - PMC - PubMed

[7] Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521:274–276. doi: 10.1038/521274a. - DOI - PubMed

[8] Baker M. Reproducibility crisis: blame it on the antibodies. Nature. 2015;521:274–276. doi: 10.1038/521274a. - DOI - PubMed

[9] Bradbury A, Plückthun A. Reproducibility: standardize antibodies used in research. Nature. 2015;518:27–29. doi: 10.1038/518027a. - DOI - PubMed

[10] Bradbury A, Plückthun A. Reproducibility: standardize antibodies used in research. Nature. 2015;518:27–29. doi: 10.1038/518027a. - DOI - PubMed

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A strong case for third-party testing

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A strong case for third-party testing

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