Pcdh19 mediates olfactory sensory neuron coalescence during postnatal stages and regeneration

Andrew P Martinez; Alexander C Chung; Suihong Huang; Adam J Bisogni; Yingxin Lin; Yue Cao; Eric O Williams; Jin Y Kim; Jean Y H Yang; David M Lin

doi:10.1016/j.isci.2023.108220

Pcdh19 mediates olfactory sensory neuron coalescence during postnatal stages and regeneration

iScience. 2023 Oct 16;26(11):108220. doi: 10.1016/j.isci.2023.108220. eCollection 2023 Nov 17.

Authors

Affiliations

¹ Department of Biomedical Sciences, Cornell University, Ithaca, NY 14850, USA.
² Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong SAR, China.
³ School of Mathematics and Statistics, F07 University of Sydney, NSW 2006, Australia.
⁴ Department of Biomedical Sciences, City University of Hong Kong, Hong Kong SAR, China.

Abstract

The mouse olfactory system regenerates constantly throughout life. While genes critical for the initial projection of olfactory sensory neurons (OSNs) to the olfactory bulb have been identified, what genes are important for maintaining the olfactory map during regeneration are still unknown. Here we show a mutation in Protocadherin 19 (Pcdh19), a cell adhesion molecule and member of the cadherin superfamily, leads to defects in OSN coalescence during regeneration. Surprisingly, lateral glomeruli were more affected and males in particular showed a more severe phenotype. Single cell analysis unexpectedly showed OSNs expressing the MOR28 odorant receptor could be subdivided into two major clusters. We showed that at least one protocadherin is differentially expressed between OSNs coalescing on the medial and lateral glomeruli. Moreover, females expressed a slightly different complement of genes from males. These features may explain the differential effects of mutating Pcdh19 on medial and lateral glomeruli in males and females.

Keywords: Molecular biology; Neuroscience; Transcriptomics.

Abstract

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