The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes

Nature. 1979 Aug 9;280(5722):468-73. doi: 10.1038/280468a0.


Ankyrin, the membrane attachment protein for human erythrocyte spectrin, is tightly linked in a 1:1 molar ratio with band 3 in detergent extracts of spectrin-depleted membranes. Ankyrin-linked band 3, which represents 10--15% of the total band 3, spans the membrane, and is nearly identical to the major band 3 by peptide analysis. Spectrin binds to solubilised ankyrin-linked band 3, but not to free band 3. A portion of band 3 remains firmly associated with detergent-extracted cytoskeletal proteins. It is concluded that a fraction of band 3 is attached to the erythrocyte cytoskeleton through association with ankyrin, which in turn is bound to spectrin.

MeSH terms

  • Chromatography, Gel
  • Erythrocyte Membrane / metabolism*
  • Erythrocytes / metabolism*
  • Humans
  • Immunosorbent Techniques
  • Isoelectric Focusing
  • Membrane Proteins / metabolism*
  • Protein Binding
  • Spectrin / metabolism*


  • Membrane Proteins
  • Spectrin