RegulonDB v12.0: a comprehensive resource of transcriptional regulation in E. coli K-12

Nucleic Acids Res. 2024 Jan 5;52(D1):D255-D264. doi: 10.1093/nar/gkad1072.


RegulonDB is a database that contains the most comprehensive corpus of knowledge of the regulation of transcription initiation of Escherichia coli K-12, including data from both classical molecular biology and high-throughput methodologies. Here, we describe biological advances since our last NAR paper of 2019. We explain the changes to satisfy FAIR requirements. We also present a full reconstruction of the RegulonDB computational infrastructure, which has significantly improved data storage, retrieval and accessibility and thus supports a more intuitive and user-friendly experience. The integration of graphical tools provides clear visual representations of genetic regulation data, facilitating data interpretation and knowledge integration. RegulonDB version 12.0 can be accessed at

MeSH terms

  • Computational Biology / methods
  • Databases, Genetic*
  • Escherichia coli K12* / genetics
  • Gene Expression Regulation, Bacterial*
  • Internet
  • Transcription, Genetic