Protocol for the integration of fiber photometry and social behavior in rodent models

Dylan J Terstege; Matthew Dawson; Naila F Jamani; Mio Tsutsui; Jonathan R Epp; Derya Sargin

doi:10.1016/j.xpro.2023.102689

Protocol for the integration of fiber photometry and social behavior in rodent models

STAR Protoc. 2023 Dec 15;4(4):102689. doi: 10.1016/j.xpro.2023.102689. Epub 2023 Nov 18.

Authors

Dylan J Terstege¹, Matthew Dawson², Naila F Jamani², Mio Tsutsui², Jonathan R Epp³, Derya Sargin⁴

Affiliations

¹ Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada. Electronic address: dylan.terstege@ucalgary.ca.
² Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.
³ Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.
⁴ Department of Psychology, University of Calgary, Calgary, AB T2N 1N4, Canada; Department of Physiology and Pharmacology, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 1N4, Canada. Electronic address: derya.sargin@ucalgary.ca.

Abstract

Fiber photometry offers insight into cell-type-specific activity underlying social interactions. We provide a protocol for the integration of fiber photometry recordings into the analysis of social behavior in rodent models. This includes considerations during surgery, notes on synchronizing fiber photometry with behavioral recordings, advice on using multi-animal behavioral tracking software, and scripts for the analysis of fiber photometry recordings. For complete details on the use and execution of this protocol, please refer to Dawson et al. (2023).¹.

Keywords: Behavior; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Photometry*
Social Behavior*
Software