Applications of Premature Chromosome Condensation technique for genetic analysis

Abstract

Cytogenetic techniques are used to detect aberrations in the genetic material and such techniques have a wide range of applications including for disease diagnosis, drug discovery and for the detection and quantification of mutagenic exposures. Although different types of cytogenetic techniques are in use, the Premature Chromosome Condensation (PCC) is one which is unique by virtue of it not requiring culture of peripheral blood mononucleate cells (PBMNCs) to detect chromatid and chromosomal aberrations. Such an advantage is useful in situations where rapid assessments of genetic damage is required, for example, during radiation exposures. PCC utilizes condensation of interphase chromatin by either biological or chemical means. The most widely used application of PCC is for biodosimetry. However, the rapidness of aberration detection has made PCC a useful technique for other applications such as for cancer diagnosis, drug-induced genotoxicity and preimplantation or assisted reproductive techniques. Also, PCC can be utilized for understanding the fundamental cellular mechanisms involved in chromatin condensation and chromosome morphologies. We present here the various approaches to obtain PCC, its applications and the endpoints which are used while using PCC as a cytogenetic technique.

Keywords: Cytogenetics; G1/G0 stage; Interphase chromatin; PCC; Primary DNA damages.