Salmonella spp. can be present in pasteurized liquid egg products because of its heat resistance or post-processing contamination, thereby representing a food safety risk. The effect of 1000 IU nisin/ml plus 20 mM disodium ethylenediaminetetraacetic acid (EDTA), two refrigerated temperatures (7 °C and 10 °C), and two inoculation levels (103 and 105 cfu/ml) were studied in the growth of S. Typhimurium in pasteurized liquid whole egg (LWE). Two mathematical models were used to fit the microbial curves. Physicochemical characteristics of LWE, such as pH and color, were assessed for 31 days at the two storage temperatures, and no significant changes (p < 0.05) were observed for most of the samples. Results showed the significant impact of temperature on microbial growth. Samples kept at 7 °C showed the decay of microbial cells during storage; meanwhile, the effect at 10 °C was shown as fast growth. The combination of nisin plus EDTA and 7 °C accelerated the decay of microbial cells during the storage showing a synergistic effect. The Weibull model described the decline of cells during the shelf-life. Meanwhile, the logistic model fitted the growth of Salmonella in LWE at 10 °C. Adding these additives to LWE, combined with the correct temperature during pasteurization and adequate conditions during the cold chain, can minimize the food safety risk related to Salmonella.
Keywords: Eggs; Food pathogens; Food safety; Hurdle technology; Natural antimicrobial; Shelf-life; Synergistic effect.
Published by Elsevier Ltd.