A nano-liposomal carrier containing p-coumaric acid for induction of targeted apoptosis on melanoma cells and kinetic modeling

Yalda Sabaghi; Farnaz PourFarzad; Leila Zolghadr; Azita Bahrami; Tahereh Shojazadeh; Alireza Farasat; Nematollah Gheibi

doi:10.1016/j.bbrc.2023.149219

A nano-liposomal carrier containing p-coumaric acid for induction of targeted apoptosis on melanoma cells and kinetic modeling

Biochem Biophys Res Commun. 2024 Jan 1:690:149219. doi: 10.1016/j.bbrc.2023.149219. Epub 2023 Nov 19.

Authors

Yalda Sabaghi¹, Farnaz PourFarzad¹, Leila Zolghadr², Azita Bahrami¹, Tahereh Shojazadeh¹, Alireza Farasat³, Nematollah Gheibi⁴

Affiliations

¹ Cellular and Molecular Research Center, Research Institute for Prevention of Non-Commuicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.
² Department of Chemistry, Imam Khomeini International University, Qazvin, Iran. Electronic address: L.zolghadr60@gmail.com.
³ Monoclnal Antibodi Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
⁴ Cellular and Molecular Research Center, Research Institute for Prevention of Non-Commuicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address: ngheibi@qums.ac.ir.

PMID: 37995451
DOI: 10.1016/j.bbrc.2023.149219

Abstract

There has been a growth in the use of plant compounds as biological products for the prevention and treatment of various diseases, including cancer. As a phenolic compound, p-Coumaric acid (p-CA) demonstrates preferrable biological effects such as anti-cancer activities. A nano-liposomal carrier containing p-CA was designed to increase the anticancer effectiveness of this compound on melanoma cells (A375). To determine the characteristics of synthesized liposomes, encapsulation efficiency was measured. In addition, the particle size was measured utilizing DLS, FTIR, and morphology examination using SEM. In vitro release was also studied through the dialysis method, while toxicity was evaluated using the MTT assay. To determine apoptotic characteristics, biotechnology tools like flow cytometry, real time PCR, and atomic force microscopy (AFM) were employed. The findings indicated that in the cells treated with the liposomal form of p-CA, the amount of elastic modulus was higher compared to its free form. Kinetic modeling indicated that the best fitting model was zero-order.

Keywords: Apoptosis; Atomic force microscopy (AFM); Kinetic modeling; Liposomal carrier; p-Coumaric acid.

MeSH terms

Apoptosis
Coumaric Acids / pharmacology
Humans
Liposomes*
Melanoma* / drug therapy

Substances

Liposomes
p-coumaric acid
Coumaric Acids