Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts

Mitchell R Harancher; Meena Sukhwani; Carlos A Castro; Michael H Hsieh; Kyle E Orwig

doi:10.1016/j.scr.2023.103257

Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts

Stem Cell Res. 2023 Dec:73:103257. doi: 10.1016/j.scr.2023.103257. Epub 2023 Nov 21.

Authors

Mitchell R Harancher¹, Meena Sukhwani², Carlos A Castro², Michael H Hsieh³, Kyle E Orwig⁴

Affiliations

¹ Molecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA.
² Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA.
³ Children's National Hospital, Washington, DC, USA.
⁴ Molecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA. Electronic address: orwigke@upmc.edu.

Abstract

Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient.

MeSH terms

Anemia, Sickle Cell* / pathology
Bone Marrow Transplantation
Child
Fibroblasts / pathology
Humans
Induced Pluripotent Stem Cells* / pathology
Male
Semen

Grants and funding

P50 HD096723/HD/NICHD NIH HHS/United States