Isolation of human liver arginase cDNA and demonstration of nonhomology between the two human arginase genes

Biochem Biophys Res Commun. 1986 Nov 26;141(1):53-9. doi: 10.1016/s0006-291x(86)80333-3.

Abstract

A human liver cDNA library was screened by colony hybridization with a rat liver arginase cDNA. The number of positive clones detected was in agreement with the estimated abundance of arginase message in liver, and the identities of several of these clones were verified by hybrid-select translation, immunoprecipitation, and competition by purified arginase. The largest of these human liver arginase cDNAs was then used to detect arginase message on northern blots at levels consistent with the activities of liver arginase in the tissues and cells studied. The absence of a hybridization signal with mRNA from a cell line expressing only human kidney arginase demonstrated the lack of homology between the two human arginase genes and indicated considerable evolutionary divergence between these two loci.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arginase / genetics*
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Gene Expression Regulation
  • Genes
  • Humans
  • Kidney / enzymology
  • Liver / enzymology*
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid

Substances

  • RNA, Messenger
  • DNA
  • Arginase