Integrating Intelligent Logic Gate Dual-Nanozyme Cascade Fluorescence Capillary Imprinted Sensors for Ultrasensitive Simultaneous Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol

Yu Chen; Kangling Tang; Qin Zhou; Xiangni Wang; Ruoyan Wang; Zhaohui Zhang

doi:10.1021/acs.analchem.3c03571

Integrating Intelligent Logic Gate Dual-Nanozyme Cascade Fluorescence Capillary Imprinted Sensors for Ultrasensitive Simultaneous Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol

Anal Chem. 2023 Dec 12;95(49):18139-18148. doi: 10.1021/acs.analchem.3c03571. Epub 2023 Nov 28.

Authors

Yu Chen^{1

2}, Kangling Tang^{1

2}, Qin Zhou^{1

2}, Xiangni Wang^{1

2}, Ruoyan Wang^{1

2}, Zhaohui Zhang^{1

2

3}

Affiliations

¹ College of Biological and Chemical Engineering, Changsha University, Changsha 410022, PR China.
² College of Chemistry and Chemical Engineering, Jishou University, Jishou,Hunan 416000, China.
³ State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.

PMID: 38013435
DOI: 10.1021/acs.analchem.3c03571

Abstract

Herein, a dual-nanozyme cascade catalysis triemission fluorescence capillary imprinted sensor integrated with intelligent logic gates was constructed for simultaneous detection of 2,4-dichlorophenoxyacetic acid (2,4-DA) and 2,4-dichlorophenol (2,4-DCP). The novel nanozyme fluorescence organic framework (Bi, Co-MOF) was grafted on the surface of Fe₃O₄ modified with histidine to form a nanozyme composite (FBM) with dual-enzyme activity, which was imprinted with 2,4-DA to prepare a fluorescence molecularly imprinted polymer (FBM@MIP). Carbon dots (CDs) coupling with FBM@MIP (FBM@MIP/CDs) was inhaled into a capillary to construct a dual-nanozyme capillary imprinted sensor directly. The FBM@MIP/CDs capillary sensor realized to detect 2,4-DA and 2,4-DCP simultaneously within a linear concentration range of 1.0 × 10^-12-1.2 × 10^-9 M and 1.0 × 10^-12-4.8 × 10^-9 M with the detection limit of 0.75 and 0.68 pM, respectively. Interestingly, a smartphone-assisted portable capillary fluorescence intelligent sensing platform was developed that can detect 2,4-DA and 2,4-DCP visually without tedious operations such as soaking and drying. Combined with a smartphone, the linear relationships between RGB ratios and concentrations of 2,4-DA and 2,4-DCP were established with the detection limit of 0.93 and 0.81 pM, respectively. The integrated logic gates provided a promising way for intelligent sensing of multiple targets simultaneously, which provided a new strategy for ultrasensitive simultaneous detection of multiple pollutants with a microvolume (18 μL/time) in complex environments.