Abstract
Reduced glutathione and other compounds with free -SH groups promoted the oxidation of low-density lipoprotein (LDL) in the absence of cells in Ham's F-10 medium. In contrast, compounds in which the thiol groups were oxidized or blocked were ineffective in oxidizing LDL. Thiol-induced modification of LDL did not occur in media lacking in redox metals. It is suggested that thiols react with redox metal, generating thiol- and oxygen-derived free radicals that promote modification of LDL.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Adhesion Molecules*
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Cysteine / pharmacology
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Endothelium / metabolism
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Glutathione / pharmacology
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In Vitro Techniques
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Lipoproteins, LDL / metabolism*
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Oxidation-Reduction / drug effects
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Rabbits
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Receptors, LDL / metabolism*
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Receptors, Scavenger
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Sulfhydryl Compounds / physiology*
Substances
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Cell Adhesion Molecules
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Lipoproteins, LDL
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Receptors, LDL
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Receptors, Scavenger
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Sulfhydryl Compounds
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Glutathione
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Cysteine