The traditional method of monitoring the oxidation and reduction of biomedical materials usually relies on electrochemical (EC) measurement techniques. Here, we demonstrate a surface plasmon resonance (SPR) method to monitor the oxidation process. Using levodopa L-dopa as the target analyte, a nanohole sensing plate is embedded in the EC electrode to enhance the oxidation signal and generate SPR. Cyclic voltammetry (CV) measurement was first conducted to understand the baseline of EC response of L-Dopa. Then, the redox reactions were simultaneously monitored through SPR measurements during the CV voltage scan. The results showed that the limit of detection using traditional CV reached 1.47 μM while using EC-SPR, the limit of detection improved to 1.23 μM. Most importantly, we found a strong correlation between CV current profiles and the SPR reflection spectra. Our results facilitate detecting electrochemical reactions using an optical probing method.
Keywords: Biosensor; Cyclic voltammetry; L-Dopa; Nanoholes array; Surface plasmon resonance.
© 2023. The Author(s).