Kinetics of arylamine N-acetyltransferase in tissues from rapid and slow acetylator mice

Carcinogenesis. 1987 Jan;8(1):133-7. doi: 10.1093/carcin/8.1.133.


Kinetic parameters for arylamine N-acetyltransferase activity in liver, blood, and bladder from C57BL/6J and A/J mouse strains were determined using an improved assay system, and some deviations were found from previously reported results. In the present studies, blood N-acetyltransferase activity with p-aminobenzoic acid and 2-aminofluorene as substrates was 20- and 10-fold greater, respectively, in C57BL/6J than in A/J mice. Urinary bladder possessed N-acetyltransferase activity for both 2-aminofluorene and p-aminobenzoic acid which differed 2-fold, and reflected the liver and blood phenotype. An apparent Km difference for 2-aminofluorene was observed between C57BL/6J and A/J liver N-acetyltransferase. Contrary to earlier studies, the liver N-acetyltransferase activity differed 3-fold between the A/J and C57BL/6J mouse strains, with either p-aminobenzoic acid or 2-aminofluorene as substrates. Dimethylsulfoxide at concentrations used in the 2-aminofluorene acetylation assay in earlier studies, inhibited the A/J liver N-acetyltransferase to a greater extent than the C57BL/6J enzyme, which may have contributed to the larger difference in liver NAT activity with 2-aminofluorene reported previously.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / metabolism*
  • Animals
  • Arylamine N-Acetyltransferase / metabolism*
  • Colorimetry
  • Fluorenes / metabolism
  • Isoenzymes / metabolism
  • Kinetics
  • Liver / enzymology
  • Mice
  • Mice, Inbred A / metabolism*
  • Mice, Inbred C57BL / metabolism*
  • Phenotype
  • Urinary Bladder / enzymology


  • Fluorenes
  • Isoenzymes
  • 2-aminofluorene
  • Acetyltransferases
  • Arylamine N-Acetyltransferase