Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: measurements using quin2

Gen Comp Endocrinol. 1986 Dec;64(3):355-61. doi: 10.1016/0016-6480(86)90068-7.

Abstract

A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 +/- 14 nM. The caudal neurosecretory peptide urotensin II at 10(-7) to 10(-6) M concentration reduced [Ca2+]c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoquinolines
  • Animals
  • Calcium / metabolism*
  • Cytoplasm / metabolism
  • Female
  • Fishes / physiology*
  • In Vitro Techniques
  • Intestinal Mucosa / metabolism
  • Male
  • Peptides / pharmacology*
  • Urotensins / pharmacology*
  • Water-Electrolyte Balance / drug effects

Substances

  • Aminoquinolines
  • Peptides
  • Urotensins
  • Quin2-acetoxymethyl ester
  • urotensin II
  • Calcium