Potential antibacterial activity and healing effect of topical administration of bone marrow and adipose mesenchymal stem cells encapsulated in collagen-fibrin hydrogel scaffold on full-thickness burn wound infection caused by Pseudomonas aeruginosa

Burns. 2023 Dec;49(8):1944-1957. doi: 10.1016/j.burns.2023.01.005. Epub 2023 Jan 16.

Abstract

Burns injuries are prone to hospital-acquired infections, and Pseudomonas aeruginosa is one of the most common causes of mortality and morbidity in patients with burn injuries. Thus, this study aimed to analyze the effects of topical treatment with bone marrow (BM-MSC) and adipose mesenchymal stem cells (AD-MSC) encapsulated in collagen and fibrin scaffolds in a Balb/c model of burn wound infection. Extraction of stem cells from adipose and bone marrow tissue of rats was performed and cells were characterized using standard methods. Then, collagen, fibrin and collagen-fibrin scaffolds were constructed and the extracted cells were encapsulated in all three scaffolds. Then, 3rd degree burn was induced in mice and 1.5 × 108 (CFU/ml) of P. aeruginosa was introduced to the burn wound. Subsequently, after 24 h of inducing wound infection, encapsulated MSCs were introduced as dressings to burn wound infection and microbial load as well as rate of wound infection healing was measured. The results of this study showed that the use of BM-MSC and AD-MSC encapsulated in collagen-fibrin scaffold reduced the bacteria load down to 54 and 21 CFU/gr, respectively (P < 0.05). Moreover, BM-MSC and AD-MSC encapsulated in collagen-fibrin showed 80% and 75% wound healing, respectively (P < 0.05). Also, we found no significant between cell origin and healing. Encapsulation of MSCs into collagen-fibrin scaffolds could be effective not only against P. aeruginosa infection, but also healing and regeneration of burn wound.

Keywords: Burn infections; Healing; Pseudomonas aeruginosa; Scaffold.

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Bacterial Agents / therapeutic use
  • Bone Marrow
  • Bone Marrow Cells
  • Burns* / drug therapy
  • Collagen / therapeutic use
  • Fibrin / therapeutic use
  • Humans
  • Hydrogels / therapeutic use
  • Mesenchymal Stem Cells*
  • Mice
  • Pseudomonas aeruginosa
  • Rats
  • Wound Healing
  • Wound Infection* / therapy

Substances

  • Hydrogels
  • Fibrin
  • Collagen
  • Anti-Bacterial Agents