Inhibitory Effects of Latilactobacillus curvatus BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic Mice

J Microbiol Biotechnol. 2024 Mar 28;34(3):589-595. doi: 10.4014/jmb.2309.09002. Epub 2023 Oct 30.


Latilactobacillus curvatus BYB3 (BYB3) is a species of lactic acid bacteria, formerly named Lactobacillus curvatus, which is isolated from kimchi. In this study, the effect of BYB3, Lactobacillus rhamnosus GG, and Lactobacillus acidophilus GP1B strain extracts at various concentrations was examined on B16F10, a mouse melanoma cell line. Cell viability was examined via MTT assay, and the results indicated that compared to the other two probiotics, BYB3 significantly decreased the total percentages of viable cells. The effects of BYB3 on cell migration and proliferation in B16F10 cells were evaluated using wound healing mobility and proliferation assays, respectively; the results indicated that BYB3 inhibits cell migration and proliferation in a concentration-dependent manner. Using human dermal fibroblast cells to investigate BYB3 extract in vivo had no effect on skin-related cells. Nonetheless, the BYB3 extract inhibited tumor growth in a mouse model, as demonstrated by liver slices. Therefore, this suggests that using BYB3 extract to inhibit melanoma may be a novel approach.

Keywords: Latilactobacillus curvatus; cell migration; melanoma B16F10 cell; proliferation.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Humans
  • Lactobacillus
  • Lactobacillus acidophilus
  • Melanoma, Experimental* / drug therapy
  • Melanoma, Experimental* / pathology
  • Mice