Direct metagenomic and amplicon-based Nanopore sequencing of French human monkeypox from clinical specimen

Microbiol Resour Announc. 2024 Jan 17;13(1):e0081123. doi: 10.1128/MRA.00811-23. Epub 2023 Dec 4.

Abstract

We report the whole-genome sequence of monkeypox virus obtained using MinION technology (Oxford Nanopore Technologies) from a French clinical specimen during the 2022 epidemic. Amplicon-based sequencing and shotgun metagenomic approaches were directly applied to the sample.

Keywords: amplicon-based sequencing; metagenomics; monkeypox virus; nanopore; next-generation sequencing.