Complete holography-based system for the identification of microparticles in water samples

J Microsc. 2024 Jan;293(1):38-58. doi: 10.1111/jmi.13249. Epub 2023 Dec 13.

Abstract

Here, we present a comprehensive holography-based system designed for detecting microparticles through microscopic holographic projections of water samples. This system is designed for researchers who may be unfamiliar with holographic technology but are engaged in microparticle research, particularly in the field of water analysis. Additionally, our innovative system can be deployed for environmental monitoring as a component of an autonomous sailboat robot. Our system's primary application is for large-scale classification of diverse microplastics that are prevalent in water bodies worldwide. This paper provides a step-by-step guide for constructing our system and outlines its entire processing pipeline, including hologram acquisition for image reconstruction.

Keywords: holographic systems; microparticles; mircoplastic; water analysis.