Localization and expression of phospholipase A2 and polyunsaturated fatty acid profile in the testis tissues of Hu sheep

Weili Liang; Yanhong Zhao; Guodong Quan; Rongyu Yao; Hua Chen; Xiuxiu Weng; Wanhong Li; Xiangpeng Yue; Fadi Li

doi:10.1016/j.anireprosci.2023.107381

Localization and expression of phospholipase A₂ and polyunsaturated fatty acid profile in the testis tissues of Hu sheep

Anim Reprod Sci. 2024 Jan:260:107381. doi: 10.1016/j.anireprosci.2023.107381. Epub 2023 Nov 23.

Authors

Weili Liang¹, Yanhong Zhao², Guodong Quan², Rongyu Yao¹, Hua Chen³, Xiuxiu Weng⁴, Wanhong Li¹, Xiangpeng Yue¹, Fadi Li⁵

Affiliations

¹ State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; Key Laboratory of Grassland Livestock Industry Innovation Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou 730020, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
² Tianzhu County Animal Breeding Research Institute, Tianzhu 733200, China.
³ Gansu Lantiantonghe Agriculture Co.,Ltd., Tianzhu 733200, China.
⁴ State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; Key Laboratory of Grassland Livestock Industry Innovation Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou 730020, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. Electronic address: wengxx@lzu.edu.cn.
⁵ State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; Key Laboratory of Grassland Livestock Industry Innovation Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou 730020, China; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China; Gansu Runmu Biological Engineering Co.,Ltd., Yongchang 737200, China.

PMID: 38056177
DOI: 10.1016/j.anireprosci.2023.107381

Abstract

The fatty acid content and the localization and expression of phospholipase A₂ (PLA₂) in the testis of Hu sheep were investigated. A total of 18 six-month-old Hu sheep were divided into small group (S, with left testis weight < 50 g), medium group (M, with left testis weight among 90-110 g), and large group (L, with left testis weight >160 g), which had six individuals each. The expression of PLA₂ in testicular tissues of different sizes was analyzed by immunohistochemistry, RT-qPCR, and Western blot. The fatty acid profile was detected by gas chromatography. Immunohistochemical labeling determined that PLA₂ protein was expressed in the Leydig and Sertoli cells of testis, and the immunohistochemical average optional density in the S group was significantly greater than the L group (P < 0.05). RT-qPCR and Western blot analysis showed that PLA₂ in the S group was greater than that in the L group (P < 0.05). Docosahexaenoic acid, ω-3 polyunsaturated fatty acid (PUFA), and total PUFA content in the testis of the L group were significantly less than those of the S and M groups (P < 0.01). This study showed that PLA₂ content in the S group was greater than that in the L group.

Keywords: Fatty acid; Hu sheep; Phospholipase A(2); Testis.

MeSH terms

Animals
Fatty Acids, Omega-3*
Fatty Acids, Unsaturated
Humans
Male
Phospholipases A2 / genetics
Sertoli Cells
Sheep
Testis*

Substances

Phospholipases A2
Fatty Acids, Unsaturated
Fatty Acids, Omega-3