Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes

Arantza Cobela-García; Ignacio Mena; Adolfo García-Sastre

doi:10.1007/978-1-0716-3533-9_3

Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes

Methods Mol Biol. 2024:2733:37-46. doi: 10.1007/978-1-0716-3533-9_3.

Authors

Arantza Cobela-García^{1

2

3}, Ignacio Mena^{4

5

6}, Adolfo García-Sastre^{1

7

8

9

10}

Affiliations

¹ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
² Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
³ Faculty of Mathematics, Informatics and Natural Sciences, University Hamburg, Hamburg, Germany.
⁴ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. imena@scripps.edu.
⁵ Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. imena@scripps.edu.
⁶ Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA. imena@scripps.edu.
⁷ Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
⁸ Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
⁹ The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
¹⁰ Department of Pathology, Molecular and Cell-BasedMedicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

PMID: 38064025
DOI: 10.1007/978-1-0716-3533-9_3

Abstract

Reverse genetics allows for the generation of recombinant infectious viruses from viral sequences or complete viral genomes cloned into plasmids. Using reverse genetics, it is then possible to introduce changes in the genome of infectious viruses for multiple applications.Newcastle disease virus (NDV) is a non-segmented, negative-sense RNA virus that has been amenable to manipulation by reverse genetics for more than two decades. Since then, recombinant NDVs have been extensively used as viral vectors to express heterologous proteins. We describe the key steps required to design and introduce an additional transcription unit in the genome of the Newcastle disease virus for the efficient expression of a heterologous gene.

Keywords: Codon optimization; Mononegavirales; Recombinant Newcastle disease virus; Reverse genetics; Virus-vectored vaccine.

MeSH terms

Animals
Chickens / genetics
Genetic Vectors / genetics
Genome, Viral
Newcastle Disease* / genetics
Newcastle disease virus / genetics
Plasmids / genetics
Viral Vaccines*

Substances

Viral Vaccines