Diagnostic value of antibody-induced procoagulant platelets in heparin-induced thrombocytopenia: communication from the ISTH SSC Subcommittee on Platelet Immunology

J Thromb Haemost. 2024 Mar;22(3):860-868. doi: 10.1016/j.jtha.2023.11.019. Epub 2023 Dec 7.


Heparin-induced thrombocytopenia (HIT) is an immune-mediated prothrombotic disorder characterized by a drop in platelet count and an increased risk of thromboembolic events. The accurate diagnosis of HIT involves clinical assessment and laboratory testing with well-characterized functional tests. Recent research has shown the potential of investigating procoagulant platelet formation induced by HIT antibodies. To successfully implement these assays in clinical laboratories, careful consideration of technical and preanalytical factors is crucial. In this communication from the SSC Platelet Immunology, we provide a consensus from experts on the use of flow cytometry in HIT diagnosis, highlighting the importance of standardized protocols.

Keywords: blood platelets; diagnostic tests routine; flow cytometry; heparin; platelet activation.

Publication types

  • Practice Guideline

MeSH terms

  • Antibodies
  • Blood Platelets
  • Communication
  • Heparin* / adverse effects
  • Humans
  • Platelet Count
  • Platelet Factor 4
  • Thrombocytopenia* / chemically induced
  • Thrombocytopenia* / diagnosis


  • Heparin
  • Antibodies
  • Platelet Factor 4