Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies

Abstract

In all cases when a bacteriophage makes a direct contact with a mammalian organism, it may challenge the mammalian immunological system. Its major consequence is the production of antibodies specific to the bacteriophage, particularly IgM, IgG, and IgA as the typical response. Here we present protocols applicable in studies of the ability of bacteriophage to induce specific antibodies; immunization to whole virions or to isolated phage proteins has been included. The protocols have been divided into three parts: purification, immunization, and detection (enzyme-linked immunosorbent assay, ELISA).

Keywords: Antibody; Bacteriophage; ELISA; IgA; IgG; IgM; Immune response; Immunization; Immunogenicity.