Synthetic Biology to Engineer Bacteriophage Genomes

Ana Rita Costa; Joana Azeredo; Diana Priscila Pires

doi:10.1007/978-1-0716-3523-0_17

Synthetic Biology to Engineer Bacteriophage Genomes

Methods Mol Biol. 2024:2734:261-277. doi: 10.1007/978-1-0716-3523-0_17.

Authors

Ana Rita Costa¹, Joana Azeredo^{2

3}, Diana Priscila Pires^{4

5}

Affiliations

¹ Department of Bionanoscience, Delft University of Technology, Delft, the Netherlands.
² CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.
³ LABBELS - Associate Laboratory, Braga, Guimarães, Portugal.
⁴ CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal. priscilapires@deb.uminho.pt.
⁵ LABBELS - Associate Laboratory, Braga, Guimarães, Portugal. priscilapires@deb.uminho.pt.

PMID: 38066375
DOI: 10.1007/978-1-0716-3523-0_17

Abstract

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered toward a wide range of applications, including pathogen control and detection, targeted drug delivery, or even assembly of new materials.In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes will be addressed: the bacteriophage recombineering of electroporated DNA (BRED) and the yeast-based phage-engineering platform.

Keywords: BRED; Bacteriophage; Bacteriophage engineering; YAC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages* / genetics
DNA
Genetic Engineering / methods
Genome, Viral
Synthetic Biology

Substances

DNA