Control of Gene Expression in the P2-related Template Coliphages. III. DNA Sequence of the Major Control Region of Phage 186

J Mol Biol. 1986 Sep 20;191(2):199-209. doi: 10.1016/0022-2836(86)90257-3.

Abstract

The PstI fragment (65.5% to 77.4%) of coliphage 186, known genetically to encode the major control genes, has been sequenced, and an analysis performed to assess coding capacity, transcription-translation signals, and to identify any other significant features. Our analysis indicates that the region encodes: seven genes, including the int and cI genes, which overlap, the late control gene B, and two genes, named CP75 and CP76, encoding potential DNA-binding proteins; a promoter pB and terminator tB for the rightward transcription of the B gene, and we predict the existence of this transcript in a lysogen; a promoter pL and terminator tL for leftward transcription that encodes the int and cI genes, and represents the presumed lysogenic transcript; a promoter pR for rightward transcription to give the presumed (early) lytic transcript that is overlapping and convergent with the lysogenic transcript; and finally, a potential operator site for repressor binding in the region of the pR promoter. Preliminary evidence is presented to support this analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Coliphages / genetics*
  • DNA, Viral*
  • Gene Expression Regulation*
  • Promoter Regions, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Ribosomes / metabolism
  • Terminator Regions, Genetic
  • Transcription, Genetic

Substances

  • DNA, Viral

Associated data

  • GENBANK/X04449