A monomeric StayGold fluorescent protein

Esther Ivorra-Molla; Dipayan Akhuli; Martin B L McAndrew; William Scott; Lokesh Kumar; Saravanan Palani; Masanori Mishima; Allister Crow; Mohan K Balasubramanian

doi:10.1038/s41587-023-02018-w

A monomeric StayGold fluorescent protein

Nat Biotechnol. 2023 Dec 11. doi: 10.1038/s41587-023-02018-w. Online ahead of print.

Authors

Affiliations

¹ Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK.
² Department of Biochemistry, Indian Institute of Science, Bangalore, India.
³ School of Life Sciences, University of Warwick, Coventry, UK.
⁴ Warwick Medical School, University of Warwick, Coventry, UK.
⁵ Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK. m.mishima@warwick.ac.uk.
⁶ School of Life Sciences, University of Warwick, Coventry, UK. allister.crow@warwick.ac.uk.
⁷ Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, UK. m.k.balasubramanian@warwick.ac.uk.

^# Contributed equally.

PMID: 38081970
DOI: 10.1038/s41587-023-02018-w

Abstract

StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

Abstract

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