Discrimination of different feed additives and poly-herbal formulations based on their untargeted phytochemical profiles
- PMID: 38082140
- DOI: 10.1007/s11306-023-02068-w
Discrimination of different feed additives and poly-herbal formulations based on their untargeted phytochemical profiles
Abstract
Introduction: Feed additives represents a valid tool in animal nutrition to improve animal performance and livestock productivity under a sustainable perspective; however, there is a paucity of information about their comprehensive metabolomic and bioactive profiles.
Objective: In this study, we tested the ability of an untargeted metabolomics approach to discriminate nine commercial feed additives and unique blends of botanical extracts used in both ruminant and non-ruminant nutrition, according to their phytochemical profiles and different in vitro bioactive properties.
Methods: An ultra-high-performance liquid chromatography coupled with Orbitrap mass spectrometry and multivariate statistics were combined to search for potential markers, in order to better discriminate the different commercial samples.
Results: Several phytochemicals were identified, namely alkaloids, phenolics, organosulfurs, and terpenoids. The polyherbal formulation Zigbir was the best source of phytochemicals, accounting for a cumulative total content of phytochemicals equal to 3.03 mg Eq./g, being particularly abundant in terpenoids, stilbenes, phenolic acids, and small-molecular-weight phenolics. Multivariate statistics allowed to group the different products in 2 bioactive subclusters. The diterpenoid andrographolide recorded the highest abundance in Zigbir and Sangrovit. The most predictive biomarkers were: piperine, isoquercitrin, 6-methylthiohexyldesulfoglucosinolate, 6-methylumbelliferone, benzoic acid, (+)-(1R,2R)-1,2-diphenylethane-1,2-diol, and piperitenone. Flavonoids were highly correlated with both in vitro antioxidant and enzyme inhibition assays.
Conclusions: Our findings provide new insights into the comprehensive phytochemical composition of commercial feed additives and blend of botanical extracts used for both ruminant and non-ruminant nutrition. A great importance of polyphenols in relation to the biological activities was detected.
Keywords: Alkaloids; Animal nutrition; Poly-herbal formulations; Polyphenols; Terpenoids; Untargeted UHPLC-HRMS.
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Similar articles
-
Phytochemical Profile, Mineral Content, and Bioactive Compounds in Leaves of Seed-Propagated Artichoke Hybrid Cultivars.Molecules. 2020 Aug 20;25(17):3795. doi: 10.3390/molecules25173795. Molecules. 2020. PMID: 32825446 Free PMC article.
-
Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia.Molecules. 2021 Jun 7;26(11):3452. doi: 10.3390/molecules26113452. Molecules. 2021. PMID: 34200152 Free PMC article.
-
A multi-analytical platform based on pressurized-liquid extraction, in vitro assays and liquid chromatography/gas chromatography coupled to high resolution mass spectrometry for food by-products valorisation. Part 2: Characterization of bioactive compounds from goldenberry (Physalis peruviana L.) calyx extracts using hyphenated techniques.J Chromatogr A. 2019 Jan 11;1584:144-154. doi: 10.1016/j.chroma.2018.11.054. Epub 2018 Nov 24. J Chromatogr A. 2019. PMID: 30579639
-
Nutritional Aspects of Ecologically Relevant Phytochemicals in Ruminant Production.Front Vet Sci. 2021 Mar 5;8:628445. doi: 10.3389/fvets.2021.628445. eCollection 2021. Front Vet Sci. 2021. PMID: 33748210 Free PMC article. Review.
-
Liquid chromatography-high resolution mass spectrometry for the analysis of phytochemicals in vegetal-derived food and beverages.Food Res Int. 2017 Oct;100(Pt 1):28-52. doi: 10.1016/j.foodres.2017.07.080. Epub 2017 Aug 2. Food Res Int. 2017. PMID: 28873689 Review.
References
-
- Abdel-Latif, M. A., Elbestawy, A. R., El-Far, A. H., Noreldin, A. E., Emam, M., Baty, R. S., Albadrani, G. M., Abdel-Daim, M. M., & El-Hamid, A. (2021). Quercetin dietary supplementation advances growth performance, gut microbiota, and intestinal mRNA expression genes in broiler chickens. Animals, 11, 2302. - PubMed - PMC
-
- Alagawany, M., Elnesr, S. S., Farag, M. R., Abd El-Hack, M. E., Barkat, R. A., Gabr, A. A., Foda, M. A., Noreldin, A. E., Khafaga, A. F., El-Sabrout, K., Elwan, H. A. M., Tiwari, R., Yatoo, M. I., Michalak, I., Di Cerbo, A., & Dhama, K. (2021). Potential role of important nutraceuticals in poultry performance and health - A comprehensive review. Research in Veterinary Science,137, 9–29. - PubMed
-
- Alagawany, M., Elnesr, S. S., Farag, M. R., Abd El-Hack, M. E., Khafaga, A. F., Taha, A. E., Tiwari, R., Yatoo, M. I., Bhatt, P., Marappan, G., & Dhama, K. (2019). Use of licorice (Glycyrrhizaglabra) herb as a feed additive in poultry: Current knowledge and prospects. Animal,9, 536.