Octadecaneuropeptide, benzodiazepine ligand, -like immunoreactivity in rat central nervous system, plasma and peripheral tissues

Neurosci Lett. 1986 Dec 12;72(2):183-8. doi: 10.1016/0304-3940(86)90077-7.

Abstract

Using a specific radioimmunoassay we have investigated the distribution of octadecaneuropeptide (a putative endogenous ligand at the benzodiazepine receptor)-like immunoreactivity (ODN-IR) in rat brain and peripheral tissues. Highest concentrations in brain were found in the hypothalamus, cerebellum and substantia nigra. Significant concentrations of ODN-IR were found in all peripheral tissues studied and in plasma. Chromatographic analysis revealed several molecular forms; one major form, indistinguishable from the synthetic peptide, was found predominantly in peripheral tissues and in plasma, while another major form, of higher molecular weight, was found in brain, peripheral tissues and plasma. Although ODN-IR was present in the synaptosomal fraction, concentrations in the microsomal fraction were higher than for other neuropeptides studied.

MeSH terms

  • Animals
  • Central Nervous System / analysis*
  • Chromatography, High Pressure Liquid
  • Diazepam Binding Inhibitor
  • Neuropeptides / analysis*
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains
  • Tissue Distribution

Substances

  • Diazepam Binding Inhibitor
  • Neuropeptides