Genome assembly and annotation of the king ratsnake, Elaphe carinata

Jiale Fan; Ruyi Huang; Diancheng Yang; Yanan Gong; Zhangbo Cui; Xinge Wang; Zicheng Su; Jing Yu; Yi Zhang; Tierui Zhang; Zhihao Jiang; Tianming Lan; He Wang; Song Huang

doi:10.46471/gigabyte.101

Genome assembly and annotation of the king ratsnake, Elaphe carinata

GigaByte. 2023 Dec 7:2023:gigabyte101. doi: 10.46471/gigabyte.101. eCollection 2023.

Authors

Jiale Fan^{1

2

3}, Ruyi Huang^{1

4

5}, Diancheng Yang^{1

5}, Yanan Gong^{1

5}, Zhangbo Cui^{1

5}, Xinge Wang^{1

5}, Zicheng Su^{1

5}, Jing Yu^{1

5}, Yi Zhang^{1

5}, Tierui Zhang^{1

5}, Zhihao Jiang^{1

5}, Tianming Lan^{2

6}, He Wang³, Song Huang^{1

5}

Affiliations

¹ Anhui Province Key Laboratory of the Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu, 241000, China.
² State Key Laboratory of Agricultural Genomics, BGI-Shenzhen, Shenzhen, 518083, China.
³ College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China.
⁴ Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China.
⁵ Huangshan Noah Biodiversity Institute, Huangshan, 245000, China.
⁶ BGI Life Science Joint Research Center, Northeast Forestry University, Harbin, 150040, China.

Abstract

The king ratsnake (Elaphe carinata) of the genus Elaphe is a common large, non-venomous snake widely distributed in Southeast and East Asia. It is an economically important farmed species. As a non-venomous snake, the king ratsnake predates venomous snakes, such as cobras and pit vipers. However, the immune and digestive mechanisms of the king ratsnake remain unclear. Despite their economic and research importance, we lack genomic resources that would benefit toxicology, phylogeography, and immunogenetics studies. Here, we used single-tube long fragment read sequencing to generate the first contiguous genome of a king ratsnake from Huangshan City, Anhui province, China. The genome size is 1.56 GB with a scaffold N50 of 6.53M. The total length of the genome is approximately 621 Mb, while the repeat content is 42.26%. Additionally, we predicted 22,339 protein-coding genes, including 22,065 with functional annotations. Our genome is a potentially useful addition to those available for snakes.

Grants and funding

Our project was financially supported by the Doctoral Research Starting Foundation of Anhui Normal University (752017), the National Natural Science Foundation of China (NSFC 31471968), and Guangdong Provincial Key Laboratory of Genome Read and Write (grant no. 2017B030301011). This work was also supported by China National GeneBank (CNGB).