Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence

F1000Res. 2023 Dec 1:12:172. doi: 10.12688/f1000research.130126.3. eCollection 2023.


Moesin is a cytoskeletal adaptor protein, involved in the modification of the actin cytoskeleton, with relevance to Alzheimer's Disease. Well characterized anti-Moesin antibodies would benefit the scientific community. In this study, we have characterized ten Moesin commercial antibodies in Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.

Keywords: MSN; Moesin; Uniprot ID P26038; Western Blot; antibody characterization; antibody validation; immunofluorescence; immunoprecipitation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies*
  • Blotting, Western
  • Cytoskeletal Proteins* / metabolism
  • Fluorescent Antibody Technique
  • Humans
  • Immunoprecipitation
  • Reproducibility of Results


  • moesin
  • Cytoskeletal Proteins
  • Antibodies