Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice

Meghan Blackwood; Qiushi Tang; Alisha M Gruntman

doi:10.1007/978-1-0716-3605-3_10

Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice

Methods Mol Biol. 2024:2750:107-112. doi: 10.1007/978-1-0716-3605-3_10.

Authors

Meghan Blackwood¹, Qiushi Tang¹, Alisha M Gruntman²

Affiliations

¹ Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
² Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA. Alisha.Gruntman@UMassMed.edu.

PMID: 38108971
DOI: 10.1007/978-1-0716-3605-3_10

Abstract

This protocol allows for the detection of a c-Myc tag on alpha-1 antitrypsin (AAT) delivered to species that already have endogenous AAT such as non-human primates allowing reliable and repeatable semi-quantitation of serum levels of AAT.

Keywords: AAT; Gene therapy; NHP; Protein levels; Serum levels.

MeSH terms

Animals
Blotting, Western
Mice
Primates*