Characteristics of CXE family of Salvia miltiorrhiza and identification of interactions between SmGID1s and SmDELLAs

Yunyun Li; Qiyue Pang; Bin Li; Yucong Fu; Mengyao Guo; Caijuan Zhang; Qian Tian; Suying Hu; Junfeng Niu; Shiqiang Wang; Donghao Wang; Zhezhi Wang

doi:10.1016/j.plaphy.2023.108140

Characteristics of CXE family of Salvia miltiorrhiza and identification of interactions between SmGID1s and SmDELLAs

Plant Physiol Biochem. 2024 Jan:206:108140. doi: 10.1016/j.plaphy.2023.108140. Epub 2023 Oct 29.

Authors

Yunyun Li¹, Qiyue Pang¹, Bin Li², Yucong Fu¹, Mengyao Guo¹, Caijuan Zhang¹, Qian Tian¹, Suying Hu¹, Junfeng Niu¹, Shiqiang Wang¹, Donghao Wang³, Zhezhi Wang⁴

Affiliations

¹ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China.
² Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China; Xi'an Botanical Garden of Shaanxi Province(Institute of Botany of Shaanxi Province), China.
³ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China. Electronic address: wangdonghao@snnu.edu.cn.
⁴ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China. Electronic address: zzwang@snnu.edu.cn.

PMID: 38134738
DOI: 10.1016/j.plaphy.2023.108140

Abstract

Carboxylesterase (CXE) is a class of hydrolases that contain an α/β folding domain, which plays critical roles in plant growth, development, and stress responses. Based on the genomic and transcriptomic data of Salvia miltiorrhiza, the SmCXE family was systematically analyzed using bioinformatics. The results revealed 34 SmCXE family members in S. miltiorrhiza, and the SmCXE family could be divided into five groups (Group I, Group II, Group III, Group IV, and Group V). Cis-regulatory elements indicated that the SmCXE promoter region contained tissue-specific and development-related, hormone-related, stress-related, and photoresponsive elements. Transcriptome analysis revealed that the expression levels of SmCXE2 were highest in roots and flowers (SmCXE8 was highest in stems and SmCXE19 was highest in leaves). Further, two GA receptors SmCXE1 (SmGID1A) and SmCXE2 (SmGID1B) were isolated from the SmCXE family, which are homologous to other plants. SmGID1A and SmGID1B have conserved HGGSF motifs and active amino acid sites (Ser-Asp-Val/IIe), which are required to maintain their GA-binding activities. SmGID1A and SmGID1B were significantly responsive to gibberellic acid (GA₃) and methyl jasmonate (MeJA) treatment. A subcellular assay revealed that SmCXE1 and SmCXE2 resided within the nucleus. SmGID1B can interact with SmDELLAs regardless of whether GA₃ exists, whereas SmGID1A can only interact with SmDELLAs in the presence of GA₃. A Further assay showed that the GRAS domain mediated the interactions between SmGID1s and SmDELLAs. This study lays a foundation for further elucidating the role of SmCXE in the growth and development of S. miltiorrhiza.

Keywords: Carboxylesterase; Differential expression; Salvia miltiorrhiza; SmDELLAs; SmGID1s.

MeSH terms

Carboxylesterase / genetics
Carboxylesterase / metabolism
Cloning, Molecular
Gene Expression Profiling
Gene Expression Regulation, Plant
Plant Proteins / metabolism
Salvia miltiorrhiza* / genetics
Salvia miltiorrhiza* / metabolism

Substances

Carboxylesterase
Plant Proteins