A high-performance-liquid-chromatographic method for the assay of coproporphyrinogen oxidase activity in rat liver

Biochem J. 1986 Oct 15;239(2):481-4. doi: 10.1042/bj2390481.


An h.p.l.c. method was developed for the assay of coproporphyrinogen oxidase activity in rat liver. The protoporphyrinogen IX formed is completely oxidized to protoporphyrin IX for separation and quantification by reversed-phase chromatography with mesoporphyrin as the internal standard. The Km of coproporphrinogen oxidase is 1.01 +/- 0.23 microM. The activities are 4.07 +/- 0.40 nmol of protoporphyrin IX/h per mg of mitochondrial protein and 224 +/- 19 nmol of protoporphyrin IX/h per g of liver tissue homogenate. The method is sensitive enough for measuring enzyme activity in small amounts of human tissue from needle biopsy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid / methods*
  • Coproporphyrinogen Oxidase / metabolism*
  • Hydrogen-Ion Concentration
  • In Vitro Techniques
  • Kinetics
  • Liver / enzymology*
  • Mitochondria, Liver / enzymology
  • Oxidation-Reduction
  • Oxidoreductases / metabolism*
  • Protoporphyrins / metabolism
  • Rats
  • Rats, Inbred Strains


  • Protoporphyrins
  • protoporphyrinogen
  • protoporphyrin IX
  • Oxidoreductases
  • Coproporphyrinogen Oxidase