Examining Phosphatases Through Immunofluorescent Microscopy

Abstract

Immunofluorescent microscopy enables the examination of cellular expression and localization of proteins. Cellular localization can often impact protein function, as certain molecular interactions occur in specific cellular compartments. Here we describe in detail the processes necessary for identifying phosphatases in the cell through immunofluorescent microscopy. Identification of phosphatase expression and localization could lead to the discovery of protein function in disease states along with potential substrates and binding partners.

Keywords: Antibody; Confocal; Immunofluorescence; Localization; Microscopy; Protein expression.