Whole-genome sequence and annotation of Penstemon davidsonii

Kate L Ostevik; Magdy Alabady; Mengrui Zhang; Mark D Rausher

doi:10.1093/g3journal/jkad296

Whole-genome sequence and annotation of Penstemon davidsonii

G3 (Bethesda). 2024 Mar 6;14(3):jkad296. doi: 10.1093/g3journal/jkad296.

Authors

Kate L Ostevik^{1

2}, Magdy Alabady³, Mengrui Zhang⁴, Mark D Rausher²

Affiliations

¹ Department of Evolution, Ecology, and Organismal Biology, University of California Riverside, Riverside, CA 92521, USA.
² Department of Biology, Duke University, Durham, NC 27708, USA.
³ Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
⁴ Department of Statistics, University of Georgia, Athens, GA 30602, USA.

Abstract

Penstemon is the most speciose flowering plant genus endemic to North America. Penstemon species' diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution. Here, we report the first full reference genome assembly and annotation for Penstemon davidsonii. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the Penstemon genus.

Keywords: Penstemon davidsonii; Davidson's beardtongue; Hi-C; PacBio Hifi; genome annotation; genome assembly.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chromosomes
Genome
High-Throughput Nucleotide Sequencing
Molecular Sequence Annotation
Penstemon* / genetics
Transcriptome

Grants and funding

DEB-1542387/NSF