Serum circPRDM5 as a novel diagnostic biomarker for acute myocardial infarction

Gene. 2024 Mar 20:899:148142. doi: 10.1016/j.gene.2024.148142. Epub 2024 Jan 5.

Abstract

Background: Circular RNA (CircRNA) is known to play an important role in cardiovascular diseases, but its use as a biomarker of acute myocardial infarction (AMI) has not been studied. This study explores the feasibility of circPRDM5 as a novel biomarker of AMI.

Methods: CircPRDM5 was screened by bioinformatics, the correct circPRDM5 primers were tested by agarose gel electrophoresis (AGE) and Sanger sequencing, and the expression level of serum circPRDM5 was detected by Quantitative Reverse Transcription-Polymerase Chain Reaction. (qRT-PCR), and the diagnostic value of circPRDM5 was analyzed by the receiver operating characteristic (ROC) curve.

Results: The expression of circPRDM5 in serum of AMI patients was significantly decreased compared with that of healthy control group and angina group (P < 0.001). The area under ROC curve of serum circPRDM5 was 0.862 [95 % CI, 0.814-0.909]. The combined diagnosis of serum circPRDM5, cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) could improve the sensitivity of diagnosing AMI. The expression level of serum circPRDM5 increased after percutaneous coronary intervention (PCI).

Conclusions: CircPRDM5 can be used as a novel biomarker for AMI, and its combination with cTnT and CK-MB can improve diagnostic value.

Keywords: AMI; Biomarker; CircPRDM5; Diagnostic.

MeSH terms

  • Biomarkers
  • Creatine Kinase, MB Form
  • Humans
  • Myocardial Infarction* / diagnosis
  • Myocardial Infarction* / genetics
  • Percutaneous Coronary Intervention*
  • ROC Curve
  • Troponin T / genetics

Substances

  • Troponin T
  • Creatine Kinase, MB Form
  • Biomarkers