Untargeted and temporal analysis of retinal lipidome in bacterial endophthalmitis

Prostaglandins Other Lipid Mediat. 2024 Apr:171:106806. doi: 10.1016/j.prostaglandins.2023.106806. Epub 2024 Jan 5.


Bacterial endophthalmitis is a blinding infectious disease typically acquired during ocular surgery. We previously reported significant alterations in retinal metabolism during Staphylococcus (S) aureus endophthalmitis. However, the changes in retinal lipid composition during endophthalmitis are unknown. Here, using a mouse model of S. aureus endophthalmitis and an untargeted lipidomic approach, we comprehensively analyzed temporal alterations in total lipids and oxylipin in retina. Our data showed a time-dependent increase in the levels of lipid classes, sphingolipids, glycerolipids, sterols, and non-esterified fatty acids, whereas levels of phospholipids decreased. Among lipid subclasses, phosphatidylcholine decreased over time. The oxylipin analysis revealed increased prostaglandin-E2, hydroxyeicosatetraenoic acids, docosahexaenoic acid, eicosapentaenoic acid, and α-linolenic acid. In-vitro studies using mouse bone marrow-derived macrophages showed increased lipid droplets and lipid-peroxide formation in response to S. aureus infection. Collectively, these findings suggest that S. aureus-infection alters the retinal lipid profile, which may contribute to the pathogenesis of bacterial endophthalmitis.

Keywords: Endophthalmitis; Inflammation; Lipidomic; Oxylipins; Retina; S. aureus.

Publication types

  • Review

MeSH terms

  • Endophthalmitis* / microbiology
  • Endophthalmitis* / pathology
  • Humans
  • Lipidomics
  • Oxylipins
  • Retina / pathology
  • Staphylococcus aureus* / physiology


  • Oxylipins