Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran

Sima Alempour Rajabi; Abdolghaffar Ownagh; Mojtaba Hadian

doi:10.1016/j.cimid.2024.102125

Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran

Comp Immunol Microbiol Infect Dis. 2024 Feb:105:102125. doi: 10.1016/j.cimid.2024.102125. Epub 2024 Jan 6.

Authors

Sima Alempour Rajabi¹, Abdolghaffar Ownagh², Mojtaba Hadian³

Affiliations

¹ Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
² Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Electronic address: a.ownagh@urmia.ac.ir.
³ Department of Clinical Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

PMID: 38199070
DOI: 10.1016/j.cimid.2024.102125

Abstract

The aim of this study was to investigate the presence and genetic characteristics of Bartonella quintana in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify B. quintana in positive samples, and the ftsZ gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the B. quintana gene, with a 95 % confidence interval of 10.71 % to 20.61 %.

Keywords: Bartonella quintana; Cat; Genomic detection; Iran; Nested-PCR; Zoonosis.

MeSH terms

Animals
Bartonella henselae* / genetics
Bartonella quintana* / genetics
Bartonella* / genetics
Cats
Genomics
Iran
Phylogeny