Homeopathic preparation of Allium sativum abrogates OxLDL mediated atherogenic events in macrophages: An invitro and in silico approach

Thangarajeswari Mohan; Abinayaa Rajkumar; Gokulprasanth Panchalingam; Debadatta Nayak; Malathi Raghunathan; Kalaiselvi Periandavan

doi:10.1016/j.jaim.2023.100850

Homeopathic preparation of Allium sativum abrogates OxLDL mediated atherogenic events in macrophages: An invitro and in silico approach

J Ayurveda Integr Med. 2024 Jan-Feb;15(1):100850. doi: 10.1016/j.jaim.2023.100850. Epub 2024 Jan 13.

Authors

Thangarajeswari Mohan¹, Abinayaa Rajkumar¹, Gokulprasanth Panchalingam¹, Debadatta Nayak², Malathi Raghunathan³, Kalaiselvi Periandavan⁴

Affiliations

¹ Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai, 600113, India.
² Central Council for Research in Homoeopathy (CCRH), New Delhi-110058, India.
³ Department of Pathology, University of Madras, Taramani Campus, Chennai, 600113, India.
⁴ Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai, 600113, India. Electronic address: pkalaiselvi@unom.ac.in.

Abstract

Background: Oxidized LDL (OxLDL), the key player in atherogenesis modulates endothelial dysfunction, initiates monocyte recruitment, accentuates foam cell formation, and flares up inflammatory and apoptotic events. Even though homeopathic preparation of Allium sativum has been proved to be an anti-inflammatory, anti-apoptotic and anti-atherogenic agent, its mechanism of action on abrogating OxLDL mediated foam cell formation is yet to be explored.

Objective: This study was designed to bring out the role of homeopathic preparation of Allium sativum in curbing OxLDL mediated cellular inflammation in IC-21 cells exposed with OxLDL.

Materials and methods: OxLDL was used to induce oxidative damage in the IC-21 macrophage cells. Assessment of inflammatory cytokines, localization of NFκB, detection of apoptosis and the in silico analysis were performed in this study.

Results: The current study portrays the efficacy of homeopathy medicine as an anti-inflammatory agent, in reducing the levels of inflammatory cytokines and its mRNA expression, suppressing the activity of NFκB and preventing apoptosis in OxLDL treated IC-21 cells.

Conclusion: To conclude, homeopathic preparation of Allium sativum 6C and 30C potencies are capable of controlling the transcriptional activity of NFκB and apoptosis in IC-21 cells exposed to OxLDL. These results implicate that Allium sativum homeopathic drug can be used as anti-inflammatory agent in reducing atherogenic events as it is capable of preventing OxLDL-mediated injury to macrophages.

Keywords: Allium sativum; Cardiovascular disease; Cytokines; OxLDL.