Optimization of ultrasound-assisted extraction of bioactive chemicals from Hemidesmus indicus (L.) R.Br. using response surface methodology and adaptive neuro-fuzzy inference system

Senthilkumar Vellur; Parasuraman Pavadai; Sureshbabu Ram Kumar Pandian; Chandrasekar Palanichamy; Shanmugampillai Jeyarajaguru Kabilan; Krishnan Sundar; Suthendran Kannan; Selvaraj Kunjiappan

doi:10.1007/s10068-023-01351-9

Optimization of ultrasound-assisted extraction of bioactive chemicals from Hemidesmus indicus (L.) R.Br. using response surface methodology and adaptive neuro-fuzzy inference system

Food Sci Biotechnol. 2023 Jun 17;33(2):327-341. doi: 10.1007/s10068-023-01351-9. eCollection 2024 Jan.

Authors

Senthilkumar Vellur¹, Parasuraman Pavadai², Sureshbabu Ram Kumar Pandian¹, Chandrasekar Palanichamy¹, Shanmugampillai Jeyarajaguru Kabilan¹, Krishnan Sundar¹, Suthendran Kannan³, Selvaraj Kunjiappan¹

Affiliations

¹ Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, 626126 India.
² Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, M S R Nagar, Bengaluru, 560054 India.
³ Department of Information Technology, Kalasalingam Academy of Research and Education, Krishnankoil, 626126 India.

Abstract

This study was designed to optimize the ultrasound-assisted extraction (UAE) of bioactive chemicals from Hemidesmus indicus (L.) R.Br. through RSM (response surface methodology) and ANFIS (adaptive neuro-fuzzy inference system). The effect of four independent parameters, methanol concentration (X₁: 55-65%), temperature (X₂: 30-40 °C), time (X₃: 15-20 min) and particle size (X₄: 0.5-1.00 mm) at five levels (- 2 ,- 1, 0, + 1, + 2) with respect to dependent parameters, total polyphenols content (TP) (y₁), total flavonoids content (TF) (y₂), %DPPHsc (y₃), %ABTSsc (y₄) and %H₂O₂sc (y₅) were selected. The optimal extraction condition was observed at X₁ = 65%, X₂ = 40 °C, X₃ = 20 min and X₄ = 0.5 mm; under this circumstance, y₁ = 352.85 mg gallic acid equivalents (GA)/g, y₂ = 300.204 mg rutin equivalents (RU)/g and their antioxidant potentials (y₃ = 81.33%, y₄ = 65.04%, and y₅ = 71.01%) has been attained. ANFIS was used to compare and confirm the optimized extraction parameter values. Further, GC-MS and LC-MS were performed to investigate the bioactive chemicals present in the optimized extract.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-023-01351-9.

Keywords: ANFIS; Hemidesmus indicus (L.) R.Br. bioactive chemicals; Optimization; RSM.

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