Cardioprotective effect of Sanguisorba minor against isoprenaline-induced myocardial infarction in rats

Azar Hosseini; Atieh Ghorbani; Mohaddeseh Sadat Alavi; Nima Forouhi; Arezoo Rajabian; Samaneh Boroumand-Noughabi; Amirhossein Sahebkar; Ali H Eid

doi:10.3389/fphar.2023.1305816

Cardioprotective effect of Sanguisorba minor against isoprenaline-induced myocardial infarction in rats

Front Pharmacol. 2023 Dec 21:14:1305816. doi: 10.3389/fphar.2023.1305816. eCollection 2023.

Authors

Azar Hosseini^{1

2}, Atieh Ghorbani³, Mohaddeseh Sadat Alavi², Nima Forouhi¹, Arezoo Rajabian⁴, Samaneh Boroumand-Noughabi⁵, Amirhossein Sahebkar^{6

7}, Ali H Eid⁸

Affiliations

¹ Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
² Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
³ Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
⁴ Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
⁵ Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
⁶ Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
⁷ Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
⁸ Department of Basic Medical Sciences, College of Medicine, QU Health, Qatar University, Doha, Qatar.

Abstract

Introduction: Oxidative stress is a major instigator of various cardiovascular diseases, including myocardial infarction (MI). Despite available drugs, there is still an increased need to look for alternative therapies or identify new bioactive compounds. Sanguisorba minor (S. minor) is a native herb characterized by its potent antioxidant activity. This study was designed to evaluate the effect of S. minor against isoprenaline-induced MI. Methods: Rats were treated with the hydro-ethanolic extract of the aerial parts of S. minor at doses of 100 or 300 mg/kg orally for 9 days. Isoprenaline was injected subcutaneously at the dose of 85 mg/kg on days 8 and 9. Then, the activities of various cardiac injury markers including cardiac troponin (cTnT), lactate dehydrogenase (LDH), creatinine kinase muscle brain (CK-MB), creatinine phosphokinase (CPK), and antioxidant enzymes in serum were determined. Malondialdehyde (MDA) and thiol content were measured in cardiac tissue, and histopathological analysis was conducted. Results: Our results show that isoprenaline increased the serum levels of cTnT, LDH, CK-MB, and CPK (p < 0.001) and elevated MDA levels (p < 0.001) in cardiac tissue. Isoprenaline also reduced superoxide dismutase (SOD), catalase, and thiol content (p < 0.001). Importantly, the extract abolished isoprenaline-induced MI by elevating SOD and catalase (p < 0.001), reducing levels of MDA, and diminishing levels of cTnT, LDH, CK-MB, and CPK cardiac markers (p < 0.001). Histopathological studies of the cardiac tissue showed isoprenaline-induced injury that was significantly attenuated by the extract. Conclusion: Our results suggest that S. minor could abrogate isoprenaline-induced cardiac toxicity due to its ability to mitigate oxidative stress.

Keywords: cardiotoxicity; herbal medicine; isoprenaline; myocardial infarction; oxidative stress; superoxide dismutase.

Grants and funding

The authors declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Vice Chancellor for Research and Technology of Mashhad University of Medical Sciences (Grant Number 990600). Open Access funding provided by the Qatar National Library.