Objective: To study the complete genome characterization of Human Astrovirus (HAstV) in Shandong Province. Methods: Stool samples from acute flaccid paralysis (AFP) surveillance in Shandong Province from 2020 to 2022 were collected, and HAstV nucleic acid was examined by real-time quantitative PCR (qPCR). Next-generation sequencing (NGS) was conducted for the positive samples to obtain complete genome sequences and identify the genotype. Homology comparison and phylogenetic analysis were performed by using BioEdit and Mega software. Results: A total of 667 samples were examined by qPCR, of which 14 were HAstV-positive (2.1%), including HAstV-1 (n=6), MLB1 (n=6), MLB2 (n=1), and VA2 (n=1). The complete genome sequences were obtained from 11 samples. The six HAstV-1 sequences of this study had 98.2% to 99.9% nt similarities with each other and 87.6% to 98.6% with those from other regions. The four MLB1 sequences of this study had 99.1% to 99.9% nt similarities with each other and 92.2% to 99.4% with those from other regions. The VA2 sequence of this study had 96.0% to 96.3% nt similarities with those from other regions. Phylogenetic analysis based on ORF2 region showed that the local HAstV-1 sequences were most closely related to Japanese strains, and had distinct topology with phylogenies based on ORF1a and ORF1b regions. Conclusion: The complete genome sequences of 11 HAstV strains are obtained, and the VA2 complete genome is found.
目的： 了解山东省人星状病毒（HAstV）的全基因组特征。 方法： 收集山东省2020—2022年急性弛缓性麻痹病例监测系统的粪便标本，采用实时荧光定量PCR检测HAstV核酸，针对阳性标本应用下一代测序技术对HAstV的全基因组序列进行测定和分析，进行型别鉴定，使用BioEdit和Mega软件进行同源性比较和系统发生学分析。 结果： 共检测了667份标本，14份为HAstV核酸阳性（2.1%），其中HAstV-1基因型6份，MLB1基因型6份，MLB2基因型1份，VA2基因型1份。共有11份标本获得了全基因组序列。其中，6株HAstV-1型本地序列之间的核苷酸相似性为98.2%~99.9%，与其他地区HAstV-1的全基因组序列的核苷酸相似性为87.6%~98.6%；4株MLB1型本地序列之间的核苷酸相似性为99.1%~99.9%，与其他地区的MLB1型全基因组序列相似性为92.2%~99.4%；1株VA2型本地序列与其他VA2型全基因组序列相似性为96.0%~96.3%。基于ORF2编码区的系统发生学结果显示，HAstV-1型本地序列与日本株具有较为密切的亲缘关系，且与基于ORF1a、ORF1b序列的系统发生树拓扑结构不一致。 结论： 共获得11株HAstV的全基因组序列，并发现了 VA2型序列。.