Ex Vivo Expansion and Homing of Human Cord Blood Hematopoietic Stem Cells

Bin Guo; Xinxin Huang; Yandan Chen; Hal E Broxmeyer

doi:10.1007/978-981-99-7471-9_6

Ex Vivo Expansion and Homing of Human Cord Blood Hematopoietic Stem Cells

Adv Exp Med Biol. 2023:1442:85-104. doi: 10.1007/978-981-99-7471-9_6.

Authors

Bin Guo^#¹, Xinxin Huang^#², Yandan Chen³, Hal E Broxmeyer⁴

Affiliations

¹ Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. jybinguo@shsmu.edu.cn.
² Xuhui Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China. xinxinhuang@fudan.edu.cn.
³ Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
⁴ Department of Microbiology and Immunology, School of Medicine, Indiana University, Indianapolis, IN, USA. hbroxmey@iupui.edu.

^# Contributed equally.

PMID: 38228960
DOI: 10.1007/978-981-99-7471-9_6

Abstract

Cord blood (CB) has been proven to be an alternative source of haematopoietic stem cells (HSCs) for clinical transplantation and has multiple advantages, including but not limited to greater HLA compatibility, lower incidence of graft-versus-host disease (GvHD), higher survival rates and lower relapse rates among patients with minimal residual disease. However, the limited number of HSCs in a single CB unit limits the wider use of CB in clinical treatment. Many efforts have been made to enhance the efficacy of CB HSC transplantation, particularly by ex vivo expansion or enhancing the homing efficiency of HSCs. In this chapter, we will document the major advances regarding human HSC ex vivo expansion and homing and will also discuss the possibility of clinical translation of such laboratory work.

Keywords: Cord blood; Ex vivo expansion; Hematopoietic stem cell; Homing.

MeSH terms

Fetal Blood
Graft vs Host Disease* / prevention & control
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells
Humans
Neoplasm Recurrence, Local