Anaerobic pig wastewater treatment commonly generates high sulfide concentrations in the treated wastewater. This study aims to apply phototrophic green sulfur bacteria (PGB) to promote sulfide removal in lighting-anaerobic digestion (lighting-AD) treating pig wastewater. Initially, batch AD tests of pig wastewater with/without PGB addition were carried out under dark (D) and light (L) conditions. The results showed that the lighting-AD with PGB gave a higher growth rate of PGB (0.056 h-1) and the highest COD/sulfide removals as compared to the dark-AD with PGB and lighting-AD solely. More experiments under various light intensities were performed in order to find an optimal intensity for PGB growth concurrently with metagenomic changes concerning treatment performance. It appeared that sulfide removal rates had increased as increasing light intensity up to 473 lx by giving the highest rate of 12.5 mg L-1 d-1 with the highest sulfur element content in the biomass. Contrastingly, many PGB species disappeared at 1350 lx exposure subsequently sharply decreasing the rate of sulfide removal. In sum, the application of low light intensities of 400-500 lx with bioaugmented PGB could promote PGB growth and activity in sulfide removal in pig wastewater in the lighting of the AD process.
Keywords: Green sulfur bacteria; Lighting anaerobic digestion; Phototrophs; Pig wastewater; Sulfide.
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.