Reconstruction of Protein/Liposome Complex

Methods Mol Biol. 2024:2766:83-91. doi: 10.1007/978-1-0716-3682-4_10.

Abstract

Most ion channels and receptors are distributed in cell membranes and are known as membrane proteins. These membrane proteins are folded in the cell membrane and become functional proteins. Here, we demonstrate a method of reconstructing membrane proteins into liposome membranes, which are commonly used as artificial cell membranes.

Keywords: Detergent; Membrane protein; Polystyrene beads; Proteoliposome; Reconstitution.

MeSH terms

  • Artificial Cells*
  • Cell Membrane
  • Liposomes*
  • Membrane Proteins
  • Membranes, Artificial

Substances

  • Liposomes
  • Membranes, Artificial
  • Membrane Proteins