Adaptation of coenzyme stimulation assays for the nutritional assessment of vitamins B1, B2 and B6 using the Cobas Bio centrifugal analyser

Ann Clin Biochem. 1987 Jan:24 ( Pt 1):41-6. doi: 10.1177/000456328702400106.

Abstract

Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at -70 degrees C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.

MeSH terms

  • Anorexia Nervosa / complications
  • Anorexia Nervosa / enzymology
  • Centrifugation
  • Clinical Enzyme Tests
  • Coenzymes / blood*
  • Erythrocytes / enzymology
  • Flavin-Adenine Dinucleotide / metabolism
  • Glutathione / metabolism
  • Glutathione Reductase / blood
  • Humans
  • Mathematics
  • NAD / metabolism
  • Pyridoxine / blood*
  • Riboflavin / blood*
  • Thiamine / blood*
  • Transketolase / blood
  • Vitamin B Deficiency / blood
  • Vitamin B Deficiency / diagnosis*

Substances

  • Coenzymes
  • NAD
  • Flavin-Adenine Dinucleotide
  • Glutathione Reductase
  • Transketolase
  • Glutathione
  • Pyridoxine
  • Riboflavin
  • Thiamine