Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans After Developmental Lead Exposure

Lucía E Fernandez-Hubeid; Paula A Albrecht; Michael Aschner; Miriam B Virgolini

doi:10.1007/978-1-0716-3625-1_15

Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans After Developmental Lead Exposure

Methods Mol Biol. 2024:2753:307-316. doi: 10.1007/978-1-0716-3625-1_15.

Authors

Lucía E Fernandez-Hubeid^{1

2}, Paula A Albrecht², Michael Aschner³, Miriam B Virgolini^{4

5}

Affiliations

¹ IFEC-CONICET, Córdoba, Argentina.
² Departamento de Farmacología Otto Orsingher, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
³ Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA.
⁴ IFEC-CONICET, Córdoba, Argentina. miriam.virgolini@unc.edu.ar.
⁵ Departamento de Farmacología Otto Orsingher, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. miriam.virgolini@unc.edu.ar.

PMID: 38285346
DOI: 10.1007/978-1-0716-3625-1_15

Abstract

The roundworm Caenorhabditis elegans (C. elegans) has become a powerful tool to evaluate the deleterious effects of early-life exposure to xenobiotics, including metals. The present chapter describes a detailed protocol for developmental lead (Pb)-exposure in C. elegans. Preliminary assays as well as the final procedure are described in detail. In addition, further protocols aimed to assess ethanol exposure at later stages of life demonstrate the impact of this drug on locomotor behavior, revealing the enduring effects that Pb can imprint on this organism when exposure occurs during development.

Keywords: Caenorhabditis elegans; Development; Ethanol; Lead exposure; Locomotion.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Intramural

MeSH terms

Animals
Biological Assay
Caenorhabditis elegans*
Ethanol / toxicity
Lead* / toxicity

Substances

Lead
Ethanol