Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments

Peter Quicke; Carmel L. Howe; Amanda J. Foust

doi:10.1007/978-1-0716-2764-8_2

Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments

Review

In: All-Optical Methods to Study Neuronal Function [Internet]. New York: Humana; 2023. Chapter 2.

2022 Jul 14.

Authors

Peter Quicke^{1

2}, Carmel L. Howe^{1

2}, Amanda J. Foust^{1

2}

Book Editor

Eirini Papagiakoumou¹

Affiliations

¹ Department of Bioengineering, Imperial College London, London, UK
² Centre for Neurotechnology, Imperial College London, London, UK

Book Affiliation

¹ Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France

Excerpt

The goal of this chapter is to establish a framework to evaluate imaging methodologies for all-optical neurophysiology experiments. This is not an exhaustive review of fluorescent indicators and imaging modalities but rather aims to distill the functional imaging principles driving the choice of both. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choice of the first constrains the choice of the second due to each’s contributions to the fluorescence budget and signal-to-noise ratio of time-varying fluorescence changes.

Sections

Publication types

Review