Huaier Regulates Oxaliplatin Resistance in Colorectal Cancer by Regulating Autophagy and Inhibiting the Wnt/β-catenin Signalling Pathway

Front Biosci (Landmark Ed). 2024 Jan 17;29(1):15. doi: 10.31083/j.fbl2901015.

Abstract

Objective: The present study aims to investigate the effect of Huaier on oxaliplatin (OXA) resistance in HCT-8 colorectal cancer (CRC) cells.

Methods: Oxaliplatin-resistant HCT-8/L CRC cells were used. The Cell Counting Kit-8, western blotting, quantitative real-time polymerase chain reaction, protein extraction kit, immunofluorescence and acridine orange staining assays were used in the study. The experiment results proved that Huaier has an influence on the Wnt/β-catenin signalling pathway, autophagy and drug resistance. The authors of the present study used chloroquine, an autophagy inhibitor and Wnt agonist 1 (a Wnt pathway agonist) to verify the present experiment.

Results: The results showed that Huaier can regulate autophagy, inhibit the Wnt/β-catenin signalling pathway and reverse the drug resistance of OXA-resistant CRC cells.

Conclusions: This study proved that Huaier can regulate autophagy, inhibit the Wnt/β-catenin signalling pathway and reverse the drug resistance of OXA-resistant CRC cells.

Keywords: Huaier; Wnt/β-catenin; autophagy; colorectal cancer; drug resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autophagy
  • Cell Line, Tumor
  • Cell Proliferation
  • Colorectal Neoplasms* / drug therapy
  • Colorectal Neoplasms* / genetics
  • Colorectal Neoplasms* / metabolism
  • Complex Mixtures*
  • Humans
  • Oxaliplatin / pharmacology
  • Trametes*
  • Wnt Signaling Pathway*
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • Oxaliplatin
  • huaier
  • beta Catenin
  • Complex Mixtures